SRS显微技术下观察RNAi干扰肥胖调节基因 这种新方法利用无需荧光标记的受激拉曼散射显微仪进行定量成像，克服了生化分析方法繁琐的色谱分析等问题，以及CARS显微方法背景信号高，结果不精确等问题，是一种简单精确，适用于高通量分析的新技术。

由于其便捷安全的操作方式，较少的占用空间，可控性好，细胞工厂已被越来越多的国内生产及科研用户接受，既有优于转瓶的优势，又没有类似生物反应器的应用限制，细胞工厂的应用将会越来越广泛，并在今后几年间迅速成为较成熟的新一代细胞培养技术。细胞工厂培养技术在疫苗行业广泛应用，如甲肝疫苗，其利用细胞工厂进行细胞培养和病毒扩增的技术已经非常成熟。

微载体培养技术是利用片状或球状微载体使细胞贴在其表面，在生物反应器中培养的技术。高密度的微载体大大增加了细胞培养的表面积，从而实现产能的扩大。但也有其缺点：劳动强度大，占地空间大，单位体积提供细胞生长的表面积小，细胞生长密度低，瓶间差异较难控制等，因而难以产业化或规模化生产。转瓶技术为传统的贴壁细胞培养技术，细胞接种在旋转的圆筒形培养器-转瓶中，培养过程中转瓶不断旋转，使细胞交替接触培养液和空气。细胞工厂（CellFactory）是一种设计精巧的细胞培养装置，在有限的空间内利用了最大限度的培养表面，从而节省了大量的厂房空间，无需进行任何厂房改造即可实现扩大产能的目的。

可用于如疫苗、单克隆抗体或生物制药等工业规模生产，特别适合于贴壁细胞，也可用于悬浮培养，在从实验室规模进行放大时不会改变细胞生长的动力学条件，可提供1，2，10和40层的规格使放大变得简单易行，低污染风险，节省空间，培养表面经测试保证最有利于细胞贴附和生长。细胞培养基 公司厂房图 显微镜下观察的细胞 工作人员进行实验 产品构想图 细胞工厂培养技术在国外已有三十多年的应用历史，近十来年在中国开始逐渐普及。监管缺失 限塑令走到交叉路口 根据限塑令规定，质检部门要对违规继续生产超薄塑料袋的，给予责令停止生产、没收违法生产的产品、没收违法所得、罚款等处罚。

某批发市场内的商店贴出销售超薄塑料袋的广告。与2009年相比，知道限塑令的消费者比例下降了近一成，消费者认为执行力度在下降，普通人对限塑令支持度也在下降，认为影响到生活便利。他们免费给袋子，我还拿篮子干嘛，这个袋子拿回家还好装垃圾用。和限塑令下发前一样，商贩们免费赠送着超薄塑料袋，市场里少有提着菜篮子来买菜的市民。

超薄塑料袋到处都是 限塑令变一纸空文 上海市普陀区宜川路菜市场，上百个摊位上，红色和白色的塑料袋成了买卖双方交易的必备品。记者花了4块钱，买了一袋红色和一袋白色塑料袋，外包装上分别写有江苏富永塑业制品厂出品和广告词为上海产品、质量保证的金山林。

工商部门要加强对超市、商场、集贸市场等商品零售场所销售、使用塑料购物袋的监督检查，对违规继续销售、使用超薄塑料购物袋等行为，要依照《中华人民共和国产品质量法》等法律法规予以查处。令人不禁要问，限塑这条路为何越走越窄？。中国每年都要消耗大量的塑料袋，这些塑料袋在提供便利的同时，造成了严重的资源浪费和环境污染，特别是超薄塑料袋成为白色污染的主要来源。卖麻辣烫的小店一到吃饭时间就生意火爆，店员熟练地将塑料袋套在碗外面，再装上各种食物，这样，顾客吃完后，就可以将剩余食物连同塑料袋一起扔掉，省得洗碗。

2007年底，国务院办公厅下发限 菜市场的商贩每天都在使用早就禁售的超薄塑料袋。上海市民李阿姨则告诉记者，她记得很清楚，当初限塑令宣布实行后，菜市场的商贩曾对她说过，把这批剩下的塑料袋用完，就不提供了，可几年过去了，情况依旧。超薄塑料袋大行其道 限塑令已成空文？ 2011-05-23 14:27 · alen 菜市场的商贩每天都在使用早就禁售的超薄塑料袋干旱无碍水稻种植 2011-05-23 14:09 · milliegu 摘要：农民并没有受干旱的严重影响，到目前已基本完成今年水稻的插秧工作，但是农业部仍告诫说缺水可能会阻碍水稻的生长。

transplanting rice into paddies(photo) Rice farmers across China fin 摘要：农民并没有受干旱的严重影响，到目前已基本完成今年水稻的插秧工作，但是农业部仍告诫说缺水可能会阻碍水稻的生长。transplanting rice into paddies(photo) Rice farmers across China finished transplanting rice into paddies this year without being seriously affected by drought, but agricultural authorities are still warning that water shortages may hinder the growth of plants. Farmers in China are expected to plant 5.87 million hectares of rice in 2011, about 106,700 hectares more than in the previous year, according to a statement released by the Ministry of Agriculture on Saturday. The amount they actually plant, though, will largely depend on the harm done by the drought conditions still present in Hubei province, the Poyang Lake areas in Jiangxi province, the Dongting Lake areas in Hunan province and the Leizhou Peninsula in Guangdong province. In Hubei alone, the dearth of water has affected about 200,000 hectares of rice paddies, the statement said. The ministry, expecting heavy rains to fall on South China until Tuesday, encouraged local agricultural departments to help in the cultivation of local paddies. From Sunday to Tuesday, drought-stricken areas along the middle and lower reaches of the Yangtze River are expected to receive from 5 mm to 30 mm of rain, and moderate to heavy rains are expected for most regions in South China, the China Meteorological Administration forecast. The current drought has been persistent. From January to May, the middle and lower reaches of the Yangtze River only saw from 40 percent to 60 percent of the rain they had seen in the same period in previous years, according to statistics from the Office of State Flood Control and Drought Relief Headquarters. By May 18, drought had caused harm to 6.54 million hectares of land in China, 2.2 million hectares of crops and 4.42 million people across the country. In many places, numbers aren't needed to indicate the severity of the drought. Hongze Lake in East China's Jiangsu province, for instance, has been so reduced that the corridors and arches of a centuries-old royal mausoleum poked up on May 19 from the water they had been submerged under for 300 years. At Honghu Lake in Hubei province, 25 percent of a 35,300-hectare lake region has dried up, causing thousands of fishing boats to run aground on the lakebed. At its deepest, the water now stands at only 30 cm, whereas it had been at between 2 and 3 meters in the past. The drought could cause ecological damage to the swamp, said Zeng Xiaodong, head of the lake's administrative office. Without water, fish, aquatic plants and birds that used to live in the lake are in danger. Zeng said local authorities have managed to protect a 333-hectare region in the lake by injecting water into it since April. In Hunan province's Luxi county, local agricultural officials have organized about 80,000 people to fight the drought by pumping water from a water reservoir. From January to May, the county saw 60 percent less rain than it had seen during the same period last year, and only managed to water about 2,867 hectares of its 9,333 hectare of parched cropland, local officials said, adding that authorities will spare no efforts to fight the drought and ensure residents have a safe water supply.

干旱无碍水稻种植 2011-05-23 14:09 · milliegu 摘要：农民并没有受干旱的严重影响，到目前已基本完成今年水稻的插秧工作，但是农业部仍告诫说缺水可能会阻碍水稻的生长。transplanting rice into paddies(photo) Rice farmers across China finished transplanting rice into paddies this year without being seriously affected by drought, but agricultural authorities are still warning that water shortages may hinder the growth of plants. Farmers in China are expected to plant 5.87 million hectares of rice in 2011, about 106,700 hectares more than in the previous year, according to a statement released by the Ministry of Agriculture on Saturday. The amount they actually plant, though, will largely depend on the harm done by the drought conditions still present in Hubei province, the Poyang Lake areas in Jiangxi province, the Dongting Lake areas in Hunan province and the Leizhou Peninsula in Guangdong province. In Hubei alone, the dearth of water has affected about 200,000 hectares of rice paddies, the statement said. The ministry, expecting heavy rains to fall on South China until Tuesday, encouraged local agricultural departments to help in the cultivation of local paddies. From Sunday to Tuesday, drought-stricken areas along the middle and lower reaches of the Yangtze River are expected to receive from 5 mm to 30 mm of rain, and moderate to heavy rains are expected for most regions in South China, the China Meteorological Administration forecast. The current drought has been persistent. From January to May, the middle and lower reaches of the Yangtze River only saw from 40 percent to 60 percent of the rain they had seen in the same period in previous years, according to statistics from the Office of State Flood Control and Drought Relief Headquarters. By May 18, drought had caused harm to 6.54 million hectares of land in China, 2.2 million hectares of crops and 4.42 million people across the country. In many places, numbers aren't needed to indicate the severity of the drought. Hongze Lake in East China's Jiangsu province, for instance, has been so reduced that the corridors and arches of a centuries-old royal mausoleum poked up on May 19 from the water they had been submerged under for 300 years. At Honghu Lake in Hubei province, 25 percent of a 35,300-hectare lake region has dried up, causing thousands of fishing boats to run aground on the lakebed. At its deepest, the water now stands at only 30 cm, whereas it had been at between 2 and 3 meters in the past. The drought could cause ecological damage to the swamp, said Zeng Xiaodong, head of the lake's administrative office. Without water, fish, aquatic plants and birds that used to live in the lake are in danger. Zeng said local authorities have managed to protect a 333-hectare region in the lake by injecting water into it since April. In Hunan province's Luxi county, local agricultural officials have organized about 80,000 people to fight the drought by pumping water from a water reservoir. From January to May, the county saw 60 percent less rain than it had seen during the same period last year, and only managed to water about 2,867 hectares of its 9,333 hectare of parched cropland, local officials said, adding that authorities will spare no efforts to fight the drought and ensure residents have a safe water supply.。

transplanting rice into paddies(photo) Rice farmers across China fin 摘要：农民并没有受干旱的严重影响，到目前已基本完成今年水稻的插秧工作，但是农业部仍告诫说缺水可能会阻碍水稻的生长但一些小作坊，原料却完全不讲究，为了节约成本有的甚至会在原料里掺加回收料。记者看到，老板这种白袋子非常薄，染色不均，有白点分布，摸起来有些粗糙。菜场里拿的超薄塑料袋点燃后不会持续燃烧，火焰边缘呈绿色并伴着黑烟，味道也很呛人。◎实验过程：超市购买的环保袋，打火机一点就着，火焰呈黄色，不断有流滴状物质产生，味道和烧蜡烛很像。塑料袋大体分两种，一种以聚乙烯为原料，一种以聚氯乙烯为原料。

而超薄袋则鲜艳很多，一捆100个只要4块钱。在日前召开的限塑令出台三周年主题研讨会上，国家发改委环资司副司长李静 环保袋 超薄袋 环保袋光谱 超薄袋光谱 ■新闻背景 限塑令将满三周年 餐馆医院或限塑 到今年6月1日，限塑令就满三周年了。

记者翻开一捆超薄塑料袋，用手在塑料袋上摸了摸，手上立马沾了一些粉末状物质。那么，这些超薄塑料袋都从哪买来的呢？南湖菜场的菜贩透露，每天都会有人到菜场卖，一个袋子只要几分钱、批发市场也能买到，更便宜。

据悉，目前我国的限塑令主要是针对超市、商场、农贸市场等。装包子也用超薄袋 你看我的袋子这么白，装吃的绝对没问题，湖西街上的一家烧饼店老板说，只有五颜六色的袋子才有毒。

他强调说，这种连卷袋含木薯成分，可以降解，因此应该不属于限塑令的范围内吧。如今，虽然限塑令将满三周年了，但在全国很多城市，一些菜市场、路边小商贩处，大批无标超薄塑料袋又大举抬头，而且被广泛用来装食品或者果蔬。记者实验 装食品的薄袋子，光谱图乱七八糟 大家都知道这些无标超薄塑料袋不好，但究竟不好在哪里，它们和环保塑料袋到底有何不同呢？这种白给的超薄塑料袋装食品后是否会传播有害物质？日前，记者来到省内检测产品质量的权威机构江苏省产品质量监督检验研究院，请专家用专业仪器给记者带去的塑料袋做了体检 实验前，先普及常识 体检前，质检院工程师向斌首先给记者普及了一些基础知识。实验3 红外光照射 结论：超薄袋光谱(右下)较乱，不能装吃的 ◎实验原理：不同物质用红外线照射会产生不同的光谱图像。

一位女士准备买点茄子，老板熟练地扯了两个青色塑料袋递给她。◎实验结论：塑料袋本身质量轻，简单依靠水中沉浮这一物理现象，对物质结构进行检测，很难判断准确。

聚氯乙烯不易燃，燃烧时冒黑烟，伴有刺鼻气味，火焰呈绿色。买白色的比较好一点，老板并未回答记者是否安全的问题，只是说白色看上去干净。

商务部商贸服务司标准规范处处长李嘉建表示，将扩大限制塑料袋使用的范围，包括餐馆、医院等场所。这袋子可以装包子吗？记者假称开包子店的。

记者看到，印有QS标志的正规环保塑料袋，外观上以淡白色或透明为主，价格在4角钱左右一个。如今他们并没有太在意市民是否拿这个装其它东西，而且也管不了。商务部称限制塑料袋使用范围将扩至餐馆医院等 2011-05-23 14:01 · alen 环保袋 超薄袋 环保袋光谱 超薄袋光谱 ■新闻背景 限塑令将满三周年 餐馆医院或限塑 到今年6月1日，限塑令就满三周年了。◎实验过程：从超薄塑料袋和带有QS标志的塑料袋上各剪下一块，然后将它们放在纯净水里，静置一分钟后，两片塑料片都浮在水面上没有下沉。

环保袋的光谱图结构单一，主要物质就是聚乙烯。记者日前对南京市场的塑料袋使用情况进行了探访 农贸市场 超薄袋五颜六色 想要几个给几个 偶尔才送一个环保袋 在南京南湖菜场，记者看到菜场里几乎每个摊位都挂着大把的塑料袋，红黄蓝绿什么颜色都有。

记者也了解到，在北京部分超市，连卷袋已不放开，需要的话得向工作人员要。一家超市的负责人告诉记者，限塑之前就有这种连卷袋了，主要是为了装一些散装食品。

市场管理员王主任对记者表示，他们曾多次通过会议、告示牌、电子屏幕等进行广泛宣传，并试图在新签订的协议书里添加不使用超薄塑料袋的条款，但遭到商贩们的一致反对，最后也只能对此睁只眼闭只眼。在很多大超市里，原先放置于散装物品或者水果边上的连卷袋，这两年使用数量明显增加，记者仔细观察了一下，有的市民甚至一撕撕几个，用连卷袋盛装洗发水等物品。